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JoVE Journal Cancer Research
Ex vivo Organoid-Modell von Adenovirus-Cre-vermittelten Gendeletionen in Urothelzellen der Maus
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Ex vivo Organoid-Modell von Adenovirus-Cre-vermittelten Gendeletionen in Urothelzellen der Maus

Article DOI: 10.3791/63855-v • 09:16 min • May 5th, 2022
May 5th, 2022 •
1, 1, 1, 2, 2, 2, 1,2
1Department of Urology, Roswell Park Comprehensive Cancer Center, 2Department of Pharmacology and Therapeutics, Roswell Park Comprehensive Cancer Center

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Dieses Protokoll beschreibt den Prozess der Erzeugung und Charakterisierung von Urothelorganoiden der Maus, die Deletionen in interessanten Genen beherbergen. Die Methoden umfassen die Gewinnung von Maus-Urothelzellen, die Ex-vivo-Transduktion mit Adenovirus-treibender Cre-Expression mit einem CMV-Promotor sowie die In-vitro- sowie In-vivo-Charakterisierung.

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Krebsforschung Ausgabe 183
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