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JoVE Journal Developmental Biology
Isolering av preadipocyter från Broiler Chick Embryos
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Isolering av preadipocyter från Broiler Chick Embryos

Article DOI: 10.3791/63861-v • 06:32 min • August 4th, 2022
August 4th, 2022 •
1, 1, 1, 1, 1
1Department of Animal Science, University of Tennessee

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Detta protokoll beskriver en enkel metod för att isolera preadipocyter från fettvävnad i broilerembryon. Denna metod möjliggör isolering med högt utbyte, primärkultur och adipogen differentiering av preadipocyter. Oil Red O-färgning och lipid / DNA-fläck mätte den adipogena förmågan hos isolerade celler inducerade med differentieringsmedier.

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Utvecklingsbiologi nummer 186
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