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Journal / Neuroscience / Sinpses de fita de imunolabeling e contagem em…
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JoVE Journal Neuroscience
Sinpses de fita de imunolabeling e contagem em jovens adultos e cócleas de Gerbil Envelhecido
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Sinpses de fita de imunolabeling e contagem em jovens adultos e cócleas de Gerbil Envelhecido

Article DOI: 10.3791/63874-v • 08:25 min • April 21st, 2022
April 21st, 2022 •
1, 1, 1
1Cluster of Excellence "Hearing4all" and Research Centre Neurosensory Science, Department of Neuroscience, School of Medicine and Health Science, Carl von Ossietzky University Oldenburg

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Um protocolo para processar a cópula gerbil jovem e envelhecida imunolabelando as estruturas sinápticas e células ciliadas anciavelmente, saciando a autofluorescência no tecido envelhecido, dissecando e estimando o comprimento da cóclea, e quantificando as sinapses em pilhas de imagens obtidas com imagens confocalas.

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Neurociência Edição 182 gerbil mongol sinapse de fita patch de glutamato postynaptic célula ciliada interna autofluorescência quencher quantificação sinapse
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