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Entrega mediada lentiviral de shRNAs para hESCs e NPCs usando polietilenimina de polímero cônico de baixo custo (PEI)

Article DOI: 10.3791/63953-v • 06:49 min • May 24th, 2022
May 24th, 2022 •
1, 1,2
1Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University, 2Zayed Center for Health Sciences, United Arab Emirates University

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Usando a polietilenimina de polímero cônico de baixo custo (PEI), produzimos partículas lentivirais para expressão estável de shRNAs em células-tronco embrionárias humanas H9 (hESCs) e células progenitoras neurais derivadas de H9 (NPCs) com alta eficiência.

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Biologia Edição 183 Lentivírus shRNA hESCs NPCs polietilenimina células HEK293T
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