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Differensierte museadipocytter i primærkultur
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Differensierte museadipocytter i primærkultur: En modell av insulinresistens

Article DOI: 10.3791/63979-v • 09:48 min • February 17th, 2023
February 17th, 2023 •
1, 1, 1, 1, 1
1Instituto de Neurobiología, Universidad Nacional Autónoma de México (UNAM)

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Denne protokollen beskriver isolering av preadipocytter fra mus fra subkutant fett, deres differensiering i modne adipocytter og induksjon av insulinresistens. Insulinvirkningen evalueres ved fosforylering/aktivering av medlemmer av insulinsignalveien gjennom western blot. Denne metoden tillater direkte bestemmelse av insulinresistens/følsomhet i primære adipocytter.

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Biologi utgave 192 preadipocyttisolering subkutant fett insulinvirkning TNF-α
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