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Journal / Developmental Biology / FACS-isolering og dyrkning af fibro-adipogene…
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Developmental Biology

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JoVE Journal Developmental Biology
FACS-isolering og dyrkning af fibro-adipogene forfædre og muskelstamceller fra uforstyrret og skadet museskeletmuskel
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Journal (Developmental Biology)
Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
Journal (Developmental Biology)
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FACS-isolering og dyrkning af fibro-adipogene forfædre og muskelstamceller fra uforstyrret og skadet museskeletmuskel

Article DOI: 10.3791/63983-v • 11:25 min • June 8th, 2022
June 8th, 2022 •
1, 2, 1
1Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS), National Institutes of Health (NIH), 2Flow Cytometry Section, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS), National Institutes of Health (NIH)

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Den præcise identifikation af fibro-adipogene stamceller (FAP'er) og muskelstamceller (MuSC'er) er afgørende for at studere deres biologiske funktion under fysiologiske og patologiske forhold. Denne protokol giver retningslinjer for isolering, rensning og kultur af FAP'er og MuSC'er fra voksne musemuskler.

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Udviklingsbiologi udgave 184 muskelfibro-adipogene forfædre muskelstamceller (MuSC'er) mesenkymale stamceller mesenkymale stromale celler FACS muskelregenerering
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