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JoVE Journal Genetics
Capture de la conformation chromosomique à travers les échelles de longueur
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Capture de la conformation chromosomique à travers les échelles de longueur

Article DOI: 10.3791/64001-v • 10:15 min • January 20th, 2023
January 20th, 2023 •
1, 1, 1,2, 1
1Department of Systems Biology, University of Massachusetts Medical School, 2Howard Hughes Medical Institute

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Hi-C 3.0 est un protocole Hi-C amélioré qui combine des agents de réticulation du formaldéhyde et du glutarate de disuccinimidyl avec un cocktail d’enzymes de restriction DpnII et DdeI pour augmenter le rapport signal sur bruit et la résolution de la détection des interactions chromatines.

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Génétique numéro 191 Capture de conformation chromosomique réticulation digestion de restriction boucles de chromatine points domaines d’association topologique compartimentation
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