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JoVE Journal Bioengineering
In vitro Rekonstitution af Actin Cytoskelettet Inde i Giant Unilamellar Vesikler
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In vitro Rekonstitution af Actin Cytoskelettet Inde i Giant Unilamellar Vesikler

Article DOI: 10.3791/64026-v • 10:19 min • August 25th, 2022
August 25th, 2022 •
1, 2,3, 1,2,3
1Department of Biomedical Engineering, Yale University, 2Systems Biology Institute, Yale University, 3Department of Physics, Yale University

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I dette manuskript demonstrerer vi de eksperimentelle teknikker til at indkapsle F-actincytoskelettet i gigantiske unilamellare lipidvesikler (også kaldet liposomer) og metoden til at danne et cortex-biomimicking F-actinlag ved den indre folder af liposommembranen.

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