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Journal / Developmental Biology / Bruk av biseksjon for å redusere mitokondrie-DNA…
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Developmental Biology

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JoVE Journal Developmental Biology
Bruk av biseksjon for å redusere mitokondrie-DNA i Bovine Oocyte
Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes
Journal (Developmental Biology)
Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes
Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle
Journal (Developmental Biology)
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Bruk av biseksjon for å redusere mitokondrie-DNA i Bovine Oocyte

Article DOI: 10.3791/64060-v • 06:15 min • July 6th, 2022
July 6th, 2022 •
*1, *1, 2, 2, 2, 3, 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2Reproductive Sciences, Beckman Center for Conservation Research, San Diego Zoo Wildlife Alliance, 3Genus PLC
* These authors contributed equally

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Her presenterer vi en protokoll for å redusere mitokondrie-DNA-kopinumrene betydelig i en storfe oocytt (P < 0,0001). Denne metoden benytter sentrifugering og biseksjon for å redusere oocytt mitokondrier betydelig og kan gi økt sjanse for utvikling i de rekonstruerte interspecies somatiske celle kjernefysiske overføring embryoer.

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Utviklingsbiologi utgave 185
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