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JoVE Journal Developmental Biology
Uso de Bisection para reduzir DNA mitocondrial no Oócito Bovino
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Uso de Bisection para reduzir DNA mitocondrial no Oócito Bovino

Article DOI: 10.3791/64060-v • 06:15 min • July 6th, 2022
July 6th, 2022 •
*1, *1, 2, 2, 2, 3, 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2Reproductive Sciences, Beckman Center for Conservation Research, San Diego Zoo Wildlife Alliance, 3Genus PLC
* These authors contributed equally

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Aqui, apresentamos um protocolo para reduzir significativamente os números de cópia de DNA mitocondrial em um oócito bovino (P < 0,0001). Este método utiliza centrifugação e biseção para reduzir substancialmente mitocôndrias oócitos e pode permitir uma maior chance de desenvolvimento nos embriões de transferência nuclear de células somáticas de células somáticas reconstruídas.

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Biologia do Desenvolvimento Edição 185
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