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Biochemistry

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JoVE Journal Biochemistry
Rekonstituering av septinmontering ved membraner for å studere biofysiske egenskaper og funksjoner
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Rekonstituering av septinmontering ved membraner for å studere biofysiske egenskaper og funksjoner

Article DOI: 10.3791/64090-v • 06:32 min • July 28th, 2022
July 28th, 2022 •
*1, *2, 1,3, 1,2,3
1Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, 2Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 3Department of Biology, University of North Carolina at Chapel Hill
* These authors contributed equally

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Cellefri rekonstituering har vært et sentralt verktøy for å forstå cytoskelettmonteringen, og arbeid i det siste tiåret har etablert tilnærminger for å studere septindynamikk i minimale systemer. Presentert her er tre komplementære metoder for å observere septinmontering i forskjellige membrankontekster: plane dobbeltlag, sfæriske støtter og stangstøtter.

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