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JoVE Journal Biology
Combinaison d’un actionneur de force magnétique 3D et d’une imagerie par fluorescence multifonctionnelle pour étudier la mécanobiologie du noyau
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Combinaison d’un actionneur de force magnétique 3D et d’une imagerie par fluorescence multifonctionnelle pour étudier la mécanobiologie du noyau

Article DOI: 10.3791/64098-v • 06:54 min • July 5th, 2022
July 5th, 2022 •
1,5, 1,5, 1, 1, 2, 3,5, 4, 5,6,7, 1, 1,5,8,9
1Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, 2Department of Materials Science and Engineering, University of Florida, 3Department of Biostatistics, University of Florida, 4Department of Biomedical Engineering, College of Engineering (COE), University of Delaware (UD), 5UF Health Cancer Center, University of Florida, 6Department of Physics, College of Liberal Arts and Sciences, University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine, University of Florida, 8J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 9Department of Physiology and Functional Genomics, University of Florida

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Cette étude présente un nouveau protocole pour appliquer directement une force mécanique sur le noyau cellulaire par le biais de microbilles magnétiques livrées dans le cytoplasme et pour effectuer simultanément l’imagerie fluorescente de cellules vivantes.

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Biologie numéro 185 Mécanobiologie du noyau force magnétique CRISPR/Cas9 mécanotransduction matière active molle protéine associée oui (YAP) imagerie fonctionnelle fluorescente traitement d’imagerie numérique optogénétique épigénétique
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