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Testa epitelial permeabilitet i fostervävnadsderiverade enteroider
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Testa epitelial permeabilitet i fostervävnadsderiverade enteroider

Article DOI: 10.3791/64108-v • 07:51 min • June 16th, 2022
June 16th, 2022 •
1, 2, 3, 3, 2
1Morsani College of Medicine, University of South Florida, 2Department of Pediatrics, Morsani College of Medicine, University of South Florida, 3Department of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of South Florida

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Detta protokoll beskriver upprättandet av enteroider, en tredimensionell tarmmodell, från fostrets tarmvävnad. Immunofluorescerande avbildning av epitelbiomarkörer användes för modellkarakterisering. Apikal exponering av lipopolysackarider, ett bakteriellt endotoxin, med hjälp av mikroinjektionsteknik inducerad epitelpermeabilitet på ett dosberoende sätt mätt genom läckage av fluorescerande dextran.

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