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Journal / Biochemistry / Enkeltpartikel-kryo-EM-dataindsamling med…
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Biochemistry

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JoVE Journal Biochemistry
Enkeltpartikel-kryo-EM-dataindsamling med scenehældning ved hjælp af Leginon
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
Journal (Biochemistry)
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
Plunge Freezing of Electron Microscopy (EM) Grids for Cryotomography (Video) | JoVE
Journal (Biochemistry)
Plunge Freezing of Electron Microscopy (EM) Grids for Cryotomography (Video) | JoVE
 
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Enkeltpartikel-kryo-EM-dataindsamling med scenehældning ved hjælp af Leginon

Article DOI: 10.3791/64136-v • 04:52 min • July 1st, 2022
July 1st, 2022 •
1, 1, 2, 2,3, 1,4,5
1Laboratory of Genetics, The Salk Institute for Biological Studies, 2Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 3Department of Chemistry and Biochemistry, University of California San Diego, 4Graduate School of Biological Sciences, Section of Molecular Biology, University of California San Diego, 5Department of Integrative Structural and Computational Biology, The Scripps Research Institute

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Denne protokol beskriver et generelt og let at implementere skema til vippet enkeltpartikeldataindsamling i kryo-EM-eksperimenter. En sådan procedure er især nyttig til opnåelse af et EM-kort af høj kvalitet for prøver, der lider af præferenceorienteringsbias på grund af overholdelse af luft-vand-grænsefladen.

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Denne måned i JoVE nummer 185
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