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Isolering af betinget udødeliggjorte museglomerulære endotelceller med fluorescerende mitokondrier

Article DOI: 10.3791/64147-v • 11:49 min • September 13th, 2022
September 13th, 2022 •
1, 1, 1, 1
1Department of Medicine, Division of Nephrology, The Icahn School of Medicine at Mount Sinai

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Artiklen beskriver metoden til isolering af betinget udødeliggjorte glomerulære endotelceller fra nyrerne hos transgene mus, der udtrykker den termolabile simianvirus 40 og fotoaktiverbare mitokondrier, PhAMudskåret. Vi beskriver proceduren for glomeruli-isolering fra hele nyrer ved hjælp af perler, fordøjelsestrin, såning og dyrkning af GECs-CD31-positive.

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Biologi udgave 187
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