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JoVE Journal
Bioengineering

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生理尺寸微流控培养装置中上皮细胞单层的制备和结构评价
 
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生理尺寸微流控培养装置中上皮细胞单层的制备和结构评价

Article DOI: 10.3791/64148-v 07:38 min July 1st, 2022
July 1st, 2022

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Summary

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所提出的协议描述了基于鬼笔环肽的丝状肌动蛋白染色技术的开发和使用,该技术与共聚焦激光扫描显微镜(CLSM)一起可视化微流体动态培养通道和传统固定孔静态培养室中的贴壁细胞层结构。这种方法有助于评估细胞层融合度、单层形成和层厚均匀性。

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