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Journal / Cancer Research / Industrialiserad, artificiell intelligensstyrd…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Industrialiserad, artificiell intelligensstyrd lasermikrodissektion för mikroskalad proteomisk analys av tumörmikromiljön
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Industrialiserad, artificiell intelligensstyrd lasermikrodissektion för mikroskalad proteomisk analys av tumörmikromiljön

Article DOI: 10.3791/64171-v • 13:01 min • June 3rd, 2022
June 3rd, 2022 •
*1,2, *1,4, 1,2, 1,2, 1,2, 1, 1,3,4, 1,2,3, 1,3,4
1Women’s Health Integrated Research Center, Gynecologic Cancer Center of Excellence, Department of Obstetrics and Gynecology, Uniformed Services University and Walter Reed National Military Medical Center, 2The Henry M. Jackson Foundation for the Advancement of Military Medicine, 3The John P. Murtha Cancer Center Research Program, Department of Surgery, Uniformed Services University, 4Women’s Health Integrated Research Center, Inova Women’s Service Line, Inova Health System
* These authors contributed equally

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Detta protokoll beskriver ett arbetsflöde med hög genomströmning för artificiell intelligens-driven segmentering av patologibekräftade regioner av intresse från färgade, tunna vävnadssektionsbilder för anrikning av histologiupplösta cellpopulationer med hjälp av lasermikrodissektion. Denna strategi inkluderar en ny algoritm som möjliggör överföring av avgränsningar som betecknar cellpopulationer av intresse direkt till lasermikroskop.

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Cancerforskning nummer 184 histopatologisk bildanalys lasermikrodissektion färskfryst vävnad formalinfixerade paraffininbäddade vävnader tryckcykelteknik tandemmassetikett kvantitativ proteomik
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