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Cocultivo y transducción de timocitos murinos en células estromales que expresan 4 similares a Delta para estudiar oncogenes en leucemia de células T
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Cocultivo y transducción de timocitos murinos en células estromales que expresan 4 similares a Delta para estudiar oncogenes en leucemia de células T

Article DOI: 10.3791/64271-v • 09:43 min • June 9th, 2023
June 9th, 2023 •
1, 1, 1,2,3, 1, 1,2,3, 4, 1, 1
1Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute, National Institutes of Health, 2Comparative Biomedical Scientist Training Program, NIH, 3Department of Pathobiology and Diagnostic Investigation, Veterinary Diagnostic Laboratory, Michigan State University, 4NCI-Frederick Laboratory Animal Sciences Program

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Este protocolo describe el aislamiento de timocitos doble negativos del timo de ratón seguido de transducción retroviral y cocultivo en el sistema de cocultivo de líneas celulares del estroma del estroma de médula ósea que expresa 4 en forma delta (OP9-DL4) para un análisis funcional adicional.

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Investigación del cáncer Número 196 Timocito OP9 linfocoyesis transducción cocultivo
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