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Fremstilling av en ikke-kardiomyocyttcellesuspensjon for enkeltcelle RNA-sekvensering fra et voksent musehjerte etter hjerteinfarkt
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Fremstilling av en ikke-kardiomyocyttcellesuspensjon for enkeltcelle RNA-sekvensering fra et voksent musehjerte etter hjerteinfarkt

Article DOI: 10.3791/64290-v • 05:54 min • February 3rd, 2023
February 3rd, 2023 •
1,2, 1,2, 1,2, *1,2, *1,2
1Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, 2Department of Cardiology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University Hangzhou
* These authors contributed equally

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Her beskriver vi en protokoll for å isolere en tilstrekkelig mengde enkeltstående ikke-kardiomyocytter med høy levedyktighet fra et musehjerte etter hjerteinfarkt (MI). Dette kan brukes til påfølgende enkeltcellesekvensering, flowcytometrianalyse og primær cellekultur.

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