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Snabb kolonibildande enhetsräkning i 96-brunnsplattformat applicerat på Drosophila-mikrobiomet
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Snabb kolonibildande enhetsräkning i 96-brunnsplattformat applicerat på Drosophila-mikrobiomet

Article DOI: 10.3791/64298-v • 12:55 min • January 13th, 2023
January 13th, 2023 •
1, 1,2
1Department of Embryology, Carnegie Institution for Science, 2Department of Biology, Johns Hopkins University

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Denna metod kvantifierar mikrobiell överflöd med hjälp av ett 96-brunnsplattformat till plattkolonibildande enheter (CFU) och appliceras på Drosophila-mikrobiomet i hela flughomogenatprover. CFUs räknas med en automatiserad bildanalysprogramvara som tillhandahålls här.

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Biologi utgåva 191
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