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Journal / Developmental Biology / Editing efficiente del genoma dei topi mediante…
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Developmental Biology

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JoVE Journal Developmental Biology
Editing efficiente del genoma dei topi mediante elettroporazione CRISPR degli zigoti
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Editing efficiente del genoma dei topi mediante elettroporazione CRISPR degli zigoti

Article DOI: 10.3791/64302-v • 07:17 min • December 16th, 2022
December 16th, 2022 •
1, 1
1Department of Biomedical Sciences, School of Veterinary Medicine, The University of Pennsylvania

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Qui, descriviamo una semplice tecnica destinata alla generazione efficiente di topi geneticamente modificati chiamata CRISPR RNP Electroporation of Zygotes (CRISPR-EZ). Questo metodo fornisce reagenti di editing mediante elettroporazione negli embrioni con un'efficienza vicina al 100%. Questo protocollo è efficace per mutazioni puntiformi, piccole inserzioni genomiche e delezioni in embrioni di mammifero.

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Biologia dello sviluppo numero 190
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