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JoVE Journal Bioengineering
Low-Dose Gamma Radiation Sterilization for Decellularized Tracheal Grafts
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Low-Dose Gamma Radiation Sterilization for Decellularized Tracheal Grafts

Article DOI: 10.3791/64432-v • 08:17 min • April 14th, 2023
April 14th, 2023 •
1, 2, 3, 4, 5, 6, 1, 2,7,8
1Thoracic Surgery Department, Hospital Universitari de la Ribera, 2Pathology Department, Universitat de Valencia Facultat de Medicina i Odontologia, 3Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, 4Radiation Oncology, Hospital Universitari de la Ribera, 5Thoracic Surgery Department, Hospitales Universitarios Virgen del Rocío, 6Department of Applied Statistics and Operations Research and Quality, Universitat Politècnica de València, 7Networking Research Center on Respiratory Diseases (CIBERER), 8INCLIVA Biomedical Research Institute

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Summary
Automatic Translation

Obtaining sterilization is essential for tracheal tissue transplant. Herein, we present a sterilization protocol using low-dose gamma irradiation that is fully tolerated by organs.

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Low-Dose Gamma Radiation Sterilization Decellularized Tracheal Grafts Non-sterile Organ Organ Sterilization Gamma Radiation Doses Effective Dose Trachea Dissection Connective Tissue Removal Inner Mucosal Layer Removal PBS Solution Osmotic Shock Cryogenization Freezing Container FBS DMSO Water Bath
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