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JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Multiparametrisches Tumor-Organoid-Wirkstoff-Screening mit Weitfeld-Live-Cell-Imaging für die Bulk- und Einzelorganoid-Analyse
Capture and Release of Viable Circulating Tumor Cells from Blood
Journal (Cancer Research)
Capture and Release of Viable Circulating Tumor Cells from Blood
Murine Model of Metastatic Liver Tumors in the Setting of Ischemia Reperfusion Injury
Journal (Cancer Research)
Murine Model of Metastatic Liver Tumors in the Setting of Ischemia Reperfusion Injury
 
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Multiparametrisches Tumor-Organoid-Wirkstoff-Screening mit Weitfeld-Live-Cell-Imaging für die Bulk- und Einzelorganoid-Analyse

Article DOI: 10.3791/64434-v • 12:41 min • December 23rd, 2022
December 23rd, 2022 •
1, 2, 1, 1, 1, 1,3, 2, 1,4, 1,4, 1,5, 1
1Center for Oncological Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON), University of Antwerp, 2Industrial Vision Lab, University of Antwerp, 3Department of Hepatobiliary Transplantation and Endocrine Surgery, University Hospital Antwerp (UZA), 4Department of Oncology, University Hospital Antwerp (UZA), 5Plasma Lab for Applications in Sustainability and Medicine ANTwerp (PLASMANT), University of Antwerp

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Dieses Protokoll beschreibt eine halbautomatische Methode für Organoid-Wirkstoff-Screenings mit mittlerem bis hohem Durchsatz und mikroskopunabhängige, automatisierte Bildanalysesoftware zur Quantifizierung und Visualisierung multiparametrischer, einzelorganoider Arzneimittelreaktionen zur Erfassung der intratumoralen Heterogenität.

Tags

Cancer Research Live-Cell Imaging Organoid Drug Screening Multiparametric Widefield Imaging Markierungsfreie Analyse Tumoroide
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