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Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins

Article DOI: 10.3791/64473-v • 10:21 min • January 13th, 2023
January 13th, 2023 •
1, 1, 1, 1
1Department of Pharmacy, University of Oslo

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Summary
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A protocol is presented for the determination of low-abundance biomarkers from dried serum samples exemplified with the biomarker progastrin-releasing peptide (ProGRP). Antibody-coated magnetic beads are used for the selective cleanup and enrichment of a proteotypic ProGRP peptide. The captured peptide is subsequently analyzed by liquid chromatography-tandem mass spectrometry.

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Microsampling Targeted Mass Spectrometry Protein Analysis Low-abundance Proteins Affinity Cleanup Protein Proteolysis Dried Microsamples Complex Samples Small Volumes Protein Biomarkers Efficient Antibodies PH Adjustment Acid Treatment Low-protein-binding Microcentrifuge Tube Magnetic Stirring Bar PH Meter Hydrochloric Acid Sodium Hydroxide
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