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Autofluorescensbilleddannelse til evaluering af rødalgerfysiologi
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Autofluorescensbilleddannelse til evaluering af rødalgerfysiologi

Article DOI: 10.3791/64533-v • 05:54 min • February 17th, 2023
February 17th, 2023 •
1, 2,3, 4
1Microscopy Core Facility, Technological Research and Scientific Area (ACTI), University of Murcia, 2Confocal Microscopy and Cellular Imaging Unit, Genetic and Molecular Medicine Department, Pediatric Institute for Rare Diseases, Hospital Sant Joan de Déu, 3Research Institute Sant Joan de Déu, Esplugues de Llobregat, Spain 4Algology Laboratory, Biology Faculty, University of Murcia, 4Algology Laboratory, Biology Faculty, University of Murcia

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Denne protokol beskriver trin-for-trin autofluorescensbilleddannelse og evaluering af fykobiliproteinændringer i rødalger baseret på spektralanalyse. Dette er en etiketfri og ikke-destruktiv metode til evaluering af cellulær tilpasning til ekstreme levesteder, når kun knappe materialer er tilgængelige, og celler vokser langsomt eller slet ikke under laboratorieforhold.

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Biologi udgave 192 autofluorescens phycobiliner klorofyl konfokal laserscanningsmikroskopi (CLSM) rødalger Chroothece
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