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JoVE Journal Neuroscience
Fraccionamiento subcelular para el aislamiento de componentes sinápticos del cerebro murino
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Fraccionamiento subcelular para el aislamiento de componentes sinápticos del cerebro murino

Article DOI: 10.3791/64574-v • 12:14 min • September 14th, 2022
September 14th, 2022 •
1,2,3, 1,2, 1,2
1Department of Neurology, Yale University, 2Department of Neuroscience, Yale University, 3Interdepartmental Neuroscience Program, Yale University

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Este protocolo presenta un método robusto y detallado para obtener sinaptosomas altamente puros, vesículas sinápticas y otras fracciones sinápticas del cerebro del ratón. Este método permite la evaluación de procesos sinápticos, incluyendo el análisis bioquímico de la localización y función de proteínas con resolución compartimental.

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Neurociencia Número 187
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