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JoVE Journal Developmental Biology
Generering av naturlige drepeceller fra humane utvidede potensielle stamceller
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Generering av naturlige drepeceller fra humane utvidede potensielle stamceller

Article DOI: 10.3791/64608-v • 06:53 min • January 13th, 2023
January 13th, 2023 •
1, 2, 2, 2,3, 2,3
1Major in Molecular Biology and Biotechnology, School of Biological Sciences, The University of Hong Kong, 2School of Biomedical Sciences, The University of Hong Kong, 3Centre for Translational Stem Cell Biology

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Den nåværende protokollen viser hvordan man skiller CD3−/CD45+CD56+-celler med mild cytotoksisitet fra humane utvidede potensielle stamceller (hEPSC) under både 3D- og 2D-kulturforhold. Dette muliggjør rutinemessig fenotypisk validering uten ødeleggelse av det komplekse mikromiljøet.

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Utviklingsbiologi utgave 191
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