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JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

변형된 효소 결합 면역흡착 분석을 사용한 인간 혈장 내 칼시토닌 유전자 관련 펩타이드(CGRP)의 검출 및 정량화

Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins

Journal (Biochemistry)

Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

Journal (Biochemistry)

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

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변형된 효소 결합 면역흡착 분석을 사용한 인간 혈장 내 칼시토닌 유전자 관련 펩타이드(CGRP)의 검출 및 정량화

Article DOI: 10.3791/64775-v • 07:14 min • June 16th, 2023

June 16th, 2023 •

Pavan S. Krishnan^1,2, Fernando T. Zamuner^1,3, Carolyn M. Jenks¹, Johnny Y. Xie⁴, Lisa Zhang⁵, Mohammed Lehar¹, Neal S. Fedarko⁶, Mariana Brait^1,3, John P. Carey¹

¹Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins University School of Medicine, ²Virginia Commonwealth University School of Medicine, ³Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, ⁴Department of Otolaryngology-Head and Neck Surgery, University of Michigan Medical School, ⁵Department of Otolaryngology-Head and Neck Surgery, The Ohio State University College of Medicine, ⁶ICTR Clinical Research Core Laboratory, Johns Hopkins University School of Medicine

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인간 혈장 내 칼시토닌 유전자 관련 펩타이드(CGRP) 농도와 관련된 발표된 데이터는 일관성이 없습니다. 이러한 불일치는 이 신경펩티드를 정량화하기 위한 표준화되고 검증된 방법론이 없기 때문일 수 있습니다. 여기에서는 인간 혈장에서 CGRP를 정제하고 정량화하기 위한 검증된 ELISA(enzyme-linked immunosorbent assay) 프로토콜을 설명합니다.

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