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JoVE Journal Neuroscience
Im lebenden Organismus Calcium-Bildgebung neuronaler Ensembles in Netzwerken primärer sensorischer Neurone in intakten dorsalen Wurzelganglien
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Im lebenden Organismus Calcium-Bildgebung neuronaler Ensembles in Netzwerken primärer sensorischer Neurone in intakten dorsalen Wurzelganglien

Article DOI: 10.3791/64826-v • 09:07 min • February 10th, 2023
February 10th, 2023 •
1, 1, 1, 1, 1,2
1Department of Oral & Maxillofacial Surgery, School of Dentistry, University of Texas Health Science Center at San Antonio, 2Programs in Integrated Biomedical Sciences, Translational Sciences, Biomedical Engineering, Radiological Sciences, University of Texas Health Science Center at San Antonio

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Dieses Protokoll beschreibt die chirurgische Exposition des dorsalen Wurzelganglions (DRG) gefolgt von GCaMP3 (genetisch kodierter Ca2+ Indikator; Grün fluoreszierendes Protein-Calmodulin-M13 Protein 3) Ca2+ Bildgebung der neuronalen Ensembles mit Pirt-GCaMP3 Mäusen unter Anwendung einer Vielzahl von Stimuli auf die ipsilaterale Hinterpfote.

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