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Journal / Immunology and Infection / Gen-Editierung von primären Rhesusaffen-B-Zellen
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Gen-Editierung von primären Rhesusaffen-B-Zellen
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Gen-Editierung von primären Rhesusaffen-B-Zellen

Article DOI: 10.3791/64858-v • 09:53 min • February 10th, 2023
February 10th, 2023 •
1, 2, 2, 3,5, 1, 1,6, 1, 2, 1,4
1Laboratory of Molecular Immunology, The Rockefeller University, 2Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 3Laboratory of Retrovirology, The Rockefeller University, 4Howard Hughes Medical Institute, The Rockefeller University, 5Laboratory of Applied Virology and Precision Medicine, King Abdullah University of Science and Technology (KAUST), 6Vaccine and Immunotherapy Center, Wistar Institute

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Wir stellen eine Methode zur Kultivierung und Gen-Editierung von primären Rhesusaffen-B-Zellen unter Verwendung von CRISPR/Cas9 und rekombinantem Adeno-assoziiertem Virus-Serotyp 6 für die Untersuchung von B-Zell-Therapien vor.

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Diesen Monat in JoVE Ausgabe 192 B-Zellen Gen-Editierung Genom-Engineering CRISPR/Cas9 Adeno-assoziiertes Virus Rhesusaffen nicht-menschliche Primaten Zelltherapie
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