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Coltura di organoidi intestinali 3D di maialino da cripte epiteliali crioconservate e creazione di monostrati cellulari
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Coltura di organoidi intestinali 3D di maialino da cripte epiteliali crioconservate e creazione di monostrati cellulari

Article DOI: 10.3791/64917-v • 08:19 min • February 10th, 2023
February 10th, 2023 •
1,2, 1, 3, 2, 4, 1, 1
1GenPhySE, Université de Toulouse, INRAE, ENVT, 2Lallemand Animal Nutrition, 3Institut NuMeCan, INRAE, INSERM, Université de Rennes, 4FG 16: Mycotic and Parasitic Agents and Mycobacteria, Robert Koch-Institute

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Qui, descriviamo un protocollo per la coltura di organoidi intestinali 3D di maiale da cripte epiteliali crioconservate. Descriviamo anche un metodo per stabilire monostrati cellulari derivati da organoidi 3D, consentendo l'accesso al lato apicale delle cellule epiteliali.

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Biologia Numero 192
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