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Journal / Biology / 使用组合的 Cre-LoxP、CRISPR-Cas9 和腺相关病毒单向导 RNA…
JoVE Journal
Biology

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JoVE Journal Biology
使用组合的 Cre-LoxP、CRISPR-Cas9 和腺相关病毒单向导 RNA 系统生成棕色脂肪特异性敲除小鼠
iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
Journal (Biology)
iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography
Journal (Biology)
Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography
 
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使用组合的 Cre-LoxP、CRISPR-Cas9 和腺相关病毒单向导 RNA 系统生成棕色脂肪特异性敲除小鼠

Article DOI: 10.3791/65083-v • 03:58 min • March 24th, 2023
March 24th, 2023 •
1, 1, 1
1Section on Integrative Physiology and Metabolism, Research Division, Joslin Diabetes Center, Harvard Medical School

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在该协议中，我们描述了利用组合的Cre-LoxP，CRISPR-Cas9和腺相关病毒（AAV）单向导RNA（sgRNA）系统生成棕色脂肪组织（BAT）特异性敲除小鼠的技术程序。所描述的步骤包括sgRNA的设计，AAV-sgRNA颗粒的制备以及将AAV显微注射到BAT叶中。

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本月在JoVE，第193期，CRISPR-Cas9，AAV-sgRNA系统，小鼠，棕色脂肪组织，显微注射
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