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Biochemistry
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JoVE Journal Biochemistry
High-speed magnetisch pincet voor nanomechanische metingen aan krachtgevoelige elementen
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High-speed magnetisch pincet voor nanomechanische metingen aan krachtgevoelige elementen

Article DOI: 10.3791/65137-v • 08:50 min • May 12th, 2023
May 12th, 2023 •
*1, *1, 1, 2,3, 2,3, 1,4
1Department of Physics, Pohang University of Science and Technology (POSTECH), 2School of Biological Sciences, Seoul National University, 3Institute for Molecular Biology and Genetics, Seoul National University, 4School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH)
* These authors contributed equally

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Hier beschrijven we een snelle magnetische pincetopstelling die nanomechanische metingen uitvoert op krachtgevoelige biomoleculen met de maximale snelheid van 1,2 kHz. We introduceren de toepassing ervan op DNA-haarspelden en SNARE-complexen als modelsystemen, maar het zal ook van toepassing zijn op andere moleculen die betrokken zijn bij mechanobiologische gebeurtenissen.

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Deze maand in JoVE nummer 195 high-speed magnetisch pincet single-molecule krachtspectroscopie DNA-haarspeld SNARE-complex
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