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Immunology and Infection

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JoVE Journal Immunology and Infection

Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research (Video) | JoVE

Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae

Journal (Immunology and Infection)

Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction

Journal (Immunology and Infection)

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction

Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research (Video) | JoVE

Multiplex Immunoassay for Borrelia-Specific IgG and IgM in Serum

Article DOI: 10.3791/65323-v • 01:59 min • July 14th, 2023

July 14th, 2023 •

Julia Häring*¹, Tanja Michel*¹, Matthias Becker¹, Daniel Junker¹, Tatia Tchitchagua², Olaf Leschnik², Berit Lange^3,4, Stefanie Castell^3,4, Gérard Krause^3,4, Monika Strengert³, Alex Dulovic¹, Nicole Schneiderhan-Marra¹

¹NMI Natural and Medical Sciences Institute at the University of Tübingen, ²Department of Neurology, Sächsisches Krankenhaus Rodewisch, ³Department of Epidemiology, Helmholtz Centre for Infection Research, ⁴German Centre for Infection Research (DZIF)

* These authors contributed equally

Tags

Simultaneous Detection Antibody Classes Multiplexed Serological Test Immunoreactivity Antigenic Determinants Antibody Isotypes Lyme Borreliosis Borrelia Species Sample Classification Immunoreactivity Against Different Antigens Anti-pathogen IgG IgM Responses Disease Progression Two-reporter Multiplex Immunoassay Borrelia-specific Immune Response Human Serum Samples Bacterial Antigens Dual-reporter Approach Analytical Performance Time And Resources Conservation Sample Size Requirements

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