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Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones

Article DOI: 10.3791/662-v • 16:30 min • February 20th, 2008
February 20th, 2008 •
1, 1, 1
1Department of Biological Sciences, Purdue University

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Aplysia californica neurons develop large growth cones in culture that are excellent for high-resolution imaging of growth cone motility and guidance. Here, we present a protocol for dissection and plating of Aplysia bag cell neurons as well as for setting up a chamber for live cell imaging.

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Neuronal Cell Cultures Aplysia High-resolution Imaging Growth Cones Guidance Cues Directional Movement Live Cell Imaging Protein Dynamics Temporal Resolution Spatial Resolution F-actin Dynamics Microtubule Dynamics Sea Hare Neurons Micromanipulation Biophysical Measurements Cytoplasmic Regions Cytoskeletal System Microinjection Growth Cone Motility Imaging Chamber
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