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JoVE Journal
Immunology and Infection

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Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut
 

Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut

Article DOI: 10.3791/2544-v 09:54 min February 14th, 2011
February 14th, 2011

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Summary

Lyme disease research studies often require generation of ticks infected with the pathogen Borrelia burgdorferi, a process that typically takes several weeks. Here we demonstrate a microinjection-based tick infection procedure that can be accomplished within hours. We also demonstrate an immunofluorescence method for in situ localization of B. burgdorferi within ticks.

Tags

Lyme Disease Pathogens Borrelia Burgdorferi Tick Gut Rapid Transfer Localization Tick-borne Murine Model Microinjection-based Procedure Spirochetes Infection Rate Pathogen Burden Attenuated Strain Biological Materials Specific Antibodies Double Stranded RNA
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