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Ex vivo Mimicry of Normal and Abnormal Human Hematopoiesis
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Bioengineering
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JoVE Journal Bioengineering
Ex vivo Mimicry of Normal and Abnormal Human Hematopoiesis

Ex vivo Mimicry of Normal and Abnormal Human Hematopoiesis

DOI:
10.3791/3654-v

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11:50 min

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April 10, 2012

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, , , , , ,
1Department of Chemical Engineering and Chemical Technology, South Kensington campus,Imperial College London , 2Department of Hematology, Northwick Park & St. Mark’s campus,Imperial College London

Chapters

  • 00:05Title
  • 01:55Scaffold Manufacturing and Bio-functionalization
  • 03:29Mononuclear Cell Isolation and Scaffold Seeding
  • 04:24In situ Cell Proliferation and Morphology: MTS Assay
  • 05:04In situ Cell Proliferation and Morphology: SEM and Cytospins
  • 06:25In situ Cell Proliferation and Morphology: Multiphoton Analysis
  • 07:39Flow Cytometric Analysis of Cellular Population
  • 08:53Representative Results
  • 10:47Conclusion

Summary

Automatic Translation

Automatic Translation

A 3D culture system for hematopoiesis is described using human cord blood and leukemic bone marrow cells. The method is based on the use of a porous synthetic polyurethane scaffold coated with extracellular matrix proteins. This scaffold is adaptable to accommodate a wide range of cells.

Tags

Ex Vivo MimicryNormal HematopoiesisAbnormal HematopoiesisHematopoietic Stem CellsBone MarrowCellular MicroenvironmentsNichesIn Vivo MicroenvironmentLeukemogenesisHematopoietic CellsStromal CellsCytokines3D Bone Marrow Culture SystemPolyurethane Scaffold

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