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JoVE Journal
Chemistry

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LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations
 

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations

Article DOI: 10.3791/4189-v 09:32 min February 4th, 2013
February 4th, 2013

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Summary

Ice binding proteins (IBPs), also known as antifreeze proteins, inhibit ice growth and are a promising additive for use in the cryopreservation of tissues. The main tool used to investigate IBPs is the nanoliter osmometer. We developed a home-designed cooling stage mounted on an optical microscope and controlled using a custom-built LabVIEW routine. The nanoliter osmometer described here manipulated the sample temperature in an ultra-sensitive manner.

Tags

LabVIEW Nanoliter Osmometer Ice-binding Proteins Antifreeze Proteins Ice Structuring Proteins Thermal Hysteresis Proteins Ice Recrystallization Inhibition Proteins Cold-adapted Organisms Fish Plants Arthropods Fungi Bacteria Adsorption Ice Crystals Gibbs-Thomson Effect Depression Thermal Hysteresis Nanoliter Osmometer Measurements Clifton Instrument Otago Instrument Osmolarity Melting Point Depression
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