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Enkelt-molekyl Imaging of Gene Regulation

Article DOI: 10.3791/50042-v • 11:31 min • March 15th, 2013
March 15th, 2013 •
1, 1,2,3, 1
1Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, 2Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 3Department of Physics, Jilin University

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Vi beskriver en fluorescens mikroskopi metode, Co-Translasjonell Aktivering av Cleavage (CoTrAC) i bildet produksjon av protein molekyler i levende celler med single-molekyl presisjon uten perturbing protein funksjonalitet. Denne metoden har blitt brukt til å følge de stokastiske uttrykk dynamikken i en transkripsjon faktor, λ repressor CI

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Biofysikk biokjemi genetikk kjemi molekylær biologi cellebiologi mikrobiologi Proteiner enkelt molekyl fluorescens protein protein uttrykk cotranslational aktivering CoTrAC cellekultur fluorescerende mikroskopi bildebehandling translasjonsforskning aktivering systembiologi
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