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Extraction and Analysis of Cortisol from Human and Monkey Hair
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JoVE Journal Biology
Extraction and Analysis of Cortisol from Human and Monkey Hair

Extraction and Analysis of Cortisol from Human and Monkey Hair

DOI:
10.3791/50882-v

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08:38 min

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January 24, 2014

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, , ,
1Department of Psychology,University of Massachusetts, Amherst, 2Neuroscience and Behavior Program,University of Massachusetts, Amherst

Chapters

  • 00:05Title
  • 01:38Sample Washing
  • 02:15Sample Grinding: Method 1 (Large Samples)
  • 02:55Cortisol (CORT) Extraction: Method 1 (Large Samples)
  • 03:41Sample Grinding: Method 2 (Small Samples)
  • 04:24CORT Extraction: Method 2 (Small Samples)
  • 05:14Solvent Evaporation, Sample Reconstitution, and CORT Assay
  • 05:51Results: Representative CORT Extraction Analysis for Human Hair Samples
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

Cortisol (CORT) accumulates in the growing hair shaft of humans and nonhuman primates. We describe methods for extracting and analyzing hair CORT with high precision and sensitivity. Measurement of hair CORT is particularly well-suited for assessing chronic stress over periods of weeks to months.

Tags

Keyword Extraction:CortisolHairExtractionAnalysisRhesus MonkeyHuman ScalpHPA SystemBiomarkerStressEnzyme ImmunoassayMethanolProtein MatrixMacaque MonkeysMarmosetsDogsPolar Bears

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