HPLC Separation, Columns, and Detection

High-performance liquid chromatography (HPLC) separates, identifies, and quantifies the parts of a complex mixture. It uses high pressure to push a liquid mobile phase through a tightly packed column. The result is a precise separation process that is useful for analyzing many samples.

In HPLC, t...

In high-performance liquid chromatography or HPLC, separation occurs in an analytical column packed with a silica-based, bonded stationary phase.

The separation of analytes takes place based on the interactions between the solid stationary phase and the liquid mobile phase.

The silica surface also consists of unreacted silanol grou...