HPLC Parts and How They Work

High-performance liquid chromatography, or HPLC, uses a set of connected parts to separate liquid samples under high pressure. The system begins with mobile phase reservoirs, which are glass bottles that hold the solvents. HPLC-grade solvents are used to keep the mobile phase as pure as possible. A degasser removes dissolved...

The major components of an HPLC instrument are a mobile phase reservoir, pump, injector, column, detector, and data system.

The mobile phase reservoir consists of one or more solvent-storage glass bottles from which the solvent is drawn through pumping systems.

In gradient elution, the solvents are mixed before they enter the colum...