A Technique to Assess the Immune Response against an Antigen by Inducing Skin Inflammation

Abstract

Source: Yao, Y., et al. Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice. J. Vis. Exp. (2016).

This video demonstrates an in vivo technique to assess a test antigen by inducing skin inflammation in a murine model. The test antigen—a hapten in an oil/acetone solution—is topically applied to the skin of the mouse ear, and inflammation of the skin is assessed after an adequate period.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Induction of Skin Inflammation in pIL1-DsRed Mice

1. Anesthetize mouse by intraperitoneal (i.p.) injection of an anesthetic cocktail containing 100 mg/kg ketamine, 10 mg/kg xylazine, and 1 mg/kg acepromazine. Adequately anesthetized mice do not show hind paw withdrawal to toe pinch.
2. Apply hair-removal cream to the dorsal surface of both ears of the mouse. Wait 30 sec to 1 min. Wipe ear surface with water-wetted cotton and allow to air-dry.
3. Measure th....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Ketamine hydrochloride	Hospira	NDC 0409-2051-05
Xylazine	LLOYD Laboratory	NADA #139-236
Acepromazine	Boehringer Ingelheim	ANADA 200-361
Hair-removal cream	Church & Dwight
Acetone	Fisher Scientific	A16P4
Oxazolone	Sigma-Aldrich	E0753