Crispr Cas9 Editing
Crispr Cas9 Editing - Related Videos
02:30
A CRISPR-Cas9 Technique for Gene Editing in T Cells
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2025
This video demonstrates an assay for performing gene editing in human T cells using the CRISPR-Cas9 technology. A mixture of primary CD4+ and CD8+ T cells is combined with a CRISPR-Cas9 ribonucleoprotein complex, targeting specific genes for knockout. Upon electroporation, the sgRNA guides Cas9 to the target DNA sequence, creating precise cuts. These cuts are then repaired by the cell's non-homologous end-joining mechanism, leading to gene knockout.
04:22
A Technique for Gene Editing in Natural Killer Cells Using CRISPR Cas9
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2025
This video demonstrates a technique for Cas9 ribonucleoprotein-mediated genetic modification of primary natural killer (NK) cells. A Cas9 ribonucleoprotein, consisting of a Cas9 endonuclease bound to a guide RNA (gRNA) formed by base pairing a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA), is introduced into primary natural killer cells via electroporation. The ribonucleoprotein targets and cleaves the host DNA at the target site, leading to gene knockout via modification of the...
11:37
QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
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Cited by 5 •
2017
Details are presented on how QTL mapping with a whole genome sequence based genetic map can be used to identify a drug resistance gene in Toxoplasma gondii and how this can be verified with the CRISPR/Cas9 system that efficiently edits a genomic target, in this case the drug resistance gene.
09:16
Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System
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Cited by 4 •
2017
This protocol describes in detail the generation of footprint-free induced pluripotent stem cells (iPSCs) from human pancreatic cells in feeder-free conditions, followed by editing using CRISPR/Cas9 ribonucleoproteins and characterization of the modified single-cell clones.
16:08
Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing
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Cited by 34 •
2018
A simple and efficient microinjection protocol for gene editing in channel catfish embryos using the CRISPR/Cas9 system is presented. In this protocol, guide RNAs and Cas9 protein were microinjected into the yolk of one-cell embryos. This protocol has been validated by knocking out two channel catfish immune-related genes.
FAQs
The targeting arrangement includes both a guide RNA matching a DNA sequence and a nearby protospacer adjacent motif, or PAM. This pairing positions Cas9 at the intended genomic site, allowing a double-strand cut. The PAM therefore helps specify where guide-directed editing can occur, making it an important consideration when selecting target sequences.
The repair response determines whether the targeted sequence is disrupted, replaced, or otherwise modified after Cas9 cuts both DNA strands. Non-homologous end joining and homology-directed repair provide different cellular routes for resolving the break. Consequently, the same cutting step can support different experimental goals, depending on the intended change at the target site.
Changing a selected gene allows investigators to examine its contribution rather than only measuring whether it is associated with a behavioral trait. Researchers can compare the resulting molecular or behavioral changes with the targeted genetic intervention, helping connect gene function to processes such as learning, stress responses, social interaction, or neurological disease.
A basic workflow begins by selecting a DNA sequence that matches a guide RNA and lies next to a suitable PAM. The guide RNA directs Cas9 to that location, where Cas9 cuts both DNA strands. Researchers then rely on cellular repair, through non-homologous end joining or homology-directed repair, to produce the planned sequence change.
This approach is useful when researchers need to test how a particular gene influences neural function or behavior. Editing can be performed in neural cells or in model organisms, enabling investigators to connect a targeted molecular change with observed behavioral outcomes. Such studies support research on learning, stress, social interaction, and neurological disease.
Behavioral applications can link a targeted genetic change with changes in molecular pathways and observable traits. The resulting evidence may help investigators evaluate gene involvement in learning, stress, or social interaction, while also examining pathways relevant to neurological disease. Its value comes from connecting events at the DNA level with neural and behavioral outcomes.