Synchrotron Instrument
Synchrotron Instrument - Related Videos
08:51
Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
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Cited by 9 •
2008
We used synchrotron X-ray tomography at the European Synchrotron Radiation Facility (ESRF) to non-invasively produce 3D tomographic datasets with a pixel-resolution of 0.7µm. Using volume rendering software, this allows the reconstruction of internal structures in their natural state without the artefacts produced by histological sectioning.
10:03
Characterization of Electrode Materials for Lithium Ion and Sodium Ion Batteries Using Synchrotron Radiation Techniques
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Cited by 9 •
2013
We describe the use of synchrotron X-ray absorption spectroscopy (XAS) and X-ray diffraction (XRD) techniques to probe details of intercalation/deintercalation processes in electrode materials for Li-ion and Na-ion batteries. Both in situ and ex situ experiments are used to understand structural behavior relevant to the operation of...
09:53
Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation
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Cited by 7 •
2012
A molecular beam coupled to tunable vacuum ultraviolet photoionization mass spectrometer at a synchrotron provides a convenient tool to explore the electronic structure of isolated gas phase molecules and clusters. Proton transfer mechanisms in DNA base dimers were elucidated with this technique.
08:46
Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
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Cited by 5 •
2016
For this study synchrotron radiation micro-tomography, a non-destructive three-dimensional imaging technique, is employed to investigate an entire microelectronic package with a cross-sectional area of 16 x 16 mm. Due to the synchrotron's high flux and brightness the sample was imaged in just 3 min with an 8.7 µm spatial resolution.
01:25
Research Instruments
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2024
Effective research relies on specialized instruments that help collect and analyze data precisely. These tools, which include surveys, interviews, observation checklists, focus groups, and mechanical devices, are each designed to capture specific types of information crucial for informed decision-making. Surveys, for instance, are commonly used to gather broad insights from large populations, such as determining public opinion on educational policies. Interviews, on the other hand, play a...
FAQs
Electron energy and magnetic guidance determine the radiation available to a synchrotron instrument. Relativistic electrons circulate in a storage ring, and their curved paths produce synchrotron light. Beamline optics then shape that radiation, while a monochromator selects a defined energy before exposure. This sequence gives biochemists a controlled probe rather than an unfiltered source.
Energy tunability matters because different measurements require different radiation conditions. By selecting the beam energy, the instrument can be adapted to structural measurements or spectroscopy, including studies of metal-site chemistry. High intensity supports measurable signals, while spatial or temporal resolution helps resolve localized features or changes under controlled conditions.
These approaches examine biochemical samples through different measurement strategies supported by the same beamline environment. Macromolecular crystallography and small-angle X-ray scattering can contribute to studies of protein structures and conformational changes, while spectroscopy can address chemical behavior such as metal-site chemistry. Together, they broaden structural and functional analysis rather than relying on one measurement type.
A typical workflow directs accelerator-generated light through beamline optics, uses a monochromator to select the desired energy, and delivers the resulting beam to the sample. A detector records the response after interaction with the sample. Researchers can perform these measurements under controlled experimental conditions, then use the recorded data to evaluate structural or chemical changes.
The measurements can reveal protein structures, conformational changes, and metal-site chemistry. These outcomes connect physical observations with biochemical function, allowing researchers to examine how a macromolecule is organized or how its state changes under defined conditions. The information may also support interpretation of ligand studies and comparisons between altered structural states.
Researchers would choose this approach when biochemical questions require unusually intense, tunable radiation or improved spatial and temporal resolution. It is relevant for investigating macromolecular structures, tracking conformational changes, examining metal-site chemistry, and evaluating structural effects under controlled conditions. The same facility can therefore support complementary structural and spectroscopic studies.
Structural measurements can show how a macromolecule is organized and how that organization changes under experimental conditions. Those observations help researchers relate structural states to molecular function and guide ligand studies. When metal-site chemistry is also examined, the resulting information adds a chemical perspective to the structural interpretation of biochemical behavior.