Biochip Reusability
Biochip Reusability - Related Videos
11:29
Reusable Hepatic Imaging Tool: A Reusable 3D-Printed Tool for Hepatic Intravital Microscopy
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2026
This protocol introduces a cutting-edge, Reusable Hepatic Imaging Tool (RHIT) for intravital microscopy (IVM) of live mice, which enhances tissue stability for rapid and acute experiments. The RHIT's 3D-printed design offers cost-effective production, scalability, and versatility, enabling prolonged observation of metabolic processes in mice of various sizes.
09:27
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
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Cited by 16 •
2016
Presented is the protocol for co-immobilizing whole-cell biocatalysts for cofactor regeneration and improved reusability, using the production of L-xylulose as an example. The cofactor regeneration is achieved by coupling two Escherichia coli strains expressing functionally complementary enzymes; the whole-cell biocatalyst immobilization is achieved by cell encapsulation in calcium alginate beads.
11:19
Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity
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Cited by 12 •
2016
This manuscript describes how to prepare fluidic biochips with Rainbow trout gill epithelial cells for use in a field portable electric cell-substrate impedance sensor. The protocol for running a rapid drinking water toxicity test with the sensor is also described.
10:28
Reusable Single Cell for Iterative Epigenomic Analyses
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2022
The present protocol describes a single-cell method for iterative epigenomic analyses using a reusable single cell. The reusable single cell allows analyses of multiple epigenetic marks in the same single cell and statistical validation of the results.
08:03
Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research
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Cited by 3 •
2013
All-solid-state ion-selective electrodes (ASSISEs) constructed from a conductive polymer (CP) transducer provide several months of functional lifetime in liquid media. Here, we describe the fabrication and calibration process of ASSISEs in a lab-on-a-chip format. The ASSISE is demonstrated to have maintained a near-Nernstian slope profile after prolonged storage in complex biological media.
FAQs
Preserving performance requires removing biological samples and contaminants without degrading the chip’s channels or sensing surfaces. Controlled washing, chemical treatment, or other decontamination steps must be matched to the biological interface being regenerated. Subsequent verification of fluid handling and signal response helps determine whether the biochip remains suitable for another experiment and continues to produce reliable measurements.
Successful reuse depends on maintaining both structural and functional performance after regeneration. The process must support accurate assays, prevent carryover from an earlier sample, and avoid damage to sensitive biological interfaces. Reliable fluid movement and signal response are therefore central criteria, because a chip that appears physically intact may still perform poorly in later analytical or biological experiments.
Carryover can allow material from a previous experiment to influence a subsequent measurement, undermining assay accuracy and repeatability. Regeneration must therefore remove biological samples and contaminants from microfluidic channels or sensing surfaces rather than merely restoring visible cleanliness. This requirement connects decontamination conditions directly to the credibility of repeated measurements and standardized bioengineering workflows.
A typical workflow begins by removing biological samples and contaminants through controlled washing, chemical treatment, or another suitable decontamination step. The regenerated chip is then checked for retained fluid-handling capability and signal response. Only after these functions remain reliable should it be redeployed, since reuse without verification could introduce carryover or performance loss into the next experiment.
Researchers should verify that regeneration has not impaired the chip’s structural or functional performance. Key checks include whether fluids still move reliably through microfluidic channels and whether sensing surfaces provide an appropriate signal response. These checks help reveal damage or incomplete decontamination before the device is used again, protecting assay accuracy and reducing uncertainty in repeated experiments.
Reusability is especially valuable when experiments require repeated measurements or standardized workflows. Redeploying a chip can reduce material consumption, experimental costs, and waste, while allowing related analyses to be performed with a consistent device platform. Its practical benefit depends on balancing those efficiencies against the need to prevent carryover and preserve sensitive biological interfaces during regeneration.