Cell Crowns
Cell Crowns - Related Videos
02:36
Crown Ethers
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2023
Crown ethers are cyclic polyethers that contain multiple oxygen atoms, usually arranged in a regular pattern. The first crown ether was synthesized by Charles Pederson while working at DuPont in 1967. For this work, Pedersen was co-awarded the 1987 Nobel Prize in Chemistry. Crown ethers are named using the formula x-crown-y, where x is the total number of atoms in the ring and y is the number of ether oxygen atoms. The term 'crown' refers to the crown-like shape that these ether molecules take.
10:50
Assessment of Immunologically Relevant Dynamic Tertiary Structural Features of the HIV-1 V3 Loop Crown R2 Sequence by ab initio Folding
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Cited by 10 •
2010
The crown region of different V3 loop sequences of the surface envelope glycoprotein (gp120) of HIV-1 can be structurally characterized in many cases by in silico folding of positions 10 to 22 of the loop using a state-of-the-art ab initio folding algorithm. Here we demonstrate the folding and evaluation of this region of the V3 loop from the R2 strain of HIV-1, a uniquely neutralization sensitive strain with puzzling functional properties.
09:27
Wind Tunnel Experiments to Study Chaparral Crown Fires
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Cited by 7 •
2017
This protocol describes wind tunnel experiments designed to study the transition of a fire from the ground to the canopy of chaparral shrubs.
14:52
Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
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Cited by 100 •
2012
The method described isolation and characterization of human Dental Pulp Stem Cells (hDPSCs) by using either enzymatic dissociation of pulp (DPSC-ED) or direct outgrowth of stem cells from pulp tissue explants (DPSC-OG). Then followed by in vitro comparative differentiation of both types of hDPSCs into odontoblasts.
08:12
Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
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Cited by 14 •
2013
Tracking of cells using MRI has gained remarkable attention in the past years. This protocol describes the labeling of dendritic cells with fluorine (19F)-rich particles, the in vivo application of these cells, and monitoring the extent of their migration to the draining lymph node with 19F/1H MRI and 19F MRS.
FAQs
The arrangement develops when cells are positioned around a central space, surface, or supporting structure under controlled culture conditions. This positioning encourages neighboring cells to contact one another while preserving an internal spatial reference. As a result, the system can produce reproducible tissue-like organization rather than relying only on randomly distributed cellular contacts.
The central feature provides the spatial cue around which cells organize. It can define the inner boundary of the arrangement and help maintain the crown-shaped architecture during culture. This geometry makes it possible to examine how cells respond to a shared spatial framework, including changes in cell-cell contact and the emergence of patterned tissue organization.
Crown geometry changes which cells are adjacent, how contacts are distributed, and how the population relates to its central feature. Those spatial differences may affect the organization and function observed in a model. In bioengineering, controlling this arrangement helps researchers examine the relationship between cellular architecture and tissue-like behavior under defined conditions.
A general workflow begins by positioning cells around a chosen central space, surface, or supporting structure. The cells are then maintained under controlled culture conditions that allow contacts and spatial patterning to develop. Researchers can subsequently examine the resulting architecture and interactions, using the organized arrangement as a structured cellular system for further study.
Researchers may choose this approach when spatial organization, rather than cell presence alone, is central to the experiment. The crown-shaped arrangement provides a defined framework for studying cell-cell interactions, tissue organization, or responses to a supporting material. It can therefore support more physiologically relevant in vitro models than systems that do not deliberately organize cells in space.
These systems can reveal how cellular arrangement relates to tissue-like architecture and function. They may also serve as structured building blocks for regenerative medicine, organoid research, and biomaterial design. By examining contacts and spatial patterning within the crown, researchers can evaluate whether an engineered cellular system reproduces features of organized living tissue.