Journal
/
Immunology and Infection
/
Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

DOI:
10.3791/50251-v

•

08:12 min

•

March 20, 2013

•

, , , , , ,
1Experimental and Clinical Research Center,A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, 2Berlin Ultrahigh Field Facility (B.U.F.F.),Max Delbrück Center for Molecular Medicine

Chapters

  • 00:05Title
  • 01:46Labeling and In vivo Application of Dendritic Cells (DC) with 19F (Fluorine)-rich Particles
  • 02:47In vivo 19F/1H (Proton) Magnetic Resonance Imaging (MRI)
  • 05:04Lymph Node Magnetic Resonance Spectroscopy (MRS)
  • 06:20Results: Quantification of 19F-labeled DC Migration In vivo using 19F MRS
  • 07:33Conclusion

Summary

Automatic Translation

Automatic Translation

Tracking of cells using MRI has gained remarkable attention in the past years. This protocol describes the labeling of dendritic cells with fluorine (19F)-rich particles, the in vivo application of these cells, and monitoring the extent of their migration to the draining lymph node with 19F/1H MRI and 19F MRS.

Tags

Keywords: Magnetic Resonance ImagingMRICell TrackingDendritic CellsFluorine-19PerfluorocarbonCell LabelingCell MigrationIn Vivo ImagingContrast AgentsUSPIOT2 EnhancementBackground-free ImagingCell QuantificationMR Spectroscopy

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50251?article_type=v

Non-invasive Imaging of Leukocyte Homing and Migration in vivo
check_url/50251?article_type=v

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
check_url/50251?article_type=v

Competitive Homing Assays to Study Gut-tropic T Cell Migration
check_url/50251?article_type=v

Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation
check_url/50251?article_type=v

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
check_url/50251?article_type=v

Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation
check_url/50251?article_type=v

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells
check_url/50251?article_type=v

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis
check_url/50251?article_type=v

A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin
check_url/50251?article_type=v

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

Read Article