Gfp-tagged Transferrin Receptor
Gfp-tagged Transferrin Receptor - Related Videos
10:41
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
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Cited by 2 •
2017
A construct encoding TMEM184A with a GFP tag at the carboxy-terminus designed for eukaryotic expression, was employed in assays designed to confirm the identification of TMEM184A as a heparin receptor in vascular cells.
09:14
Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
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Cited by 5 •
2012
In this protocol, we update recent progress in imaging Ca2+ signals of GFP-tagged neurons in brain tissue slices using a red fluorescent Ca2+ indicator dye.
11:57
Using an α-Bungarotoxin Binding Site Tag to Study GABA A Receptor Membrane Localization and Trafficking
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Cited by 5 •
2014
Here we demonstrate the use of fluorescent Alexa dye coupled to α-bungarotoxin to measure GABA A receptor surface localization and endocytosis in hippocampal neurons. Through the use of constructs bearing a short extracellular tag that binds α-bungarotoxin, analysis of plasma membrane protein endocytic trafficking can be achieved.
05:00
Monitoring the Arrival of GFP-Tagged Surface Proteins to the Trans-Golgi Network Using Functionalized Nanobodies
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2025
This video demonstrates a method to study the uptake and retrograde transport of functionalized nanobodies to understand their intracellular trafficking pathways. By introducing radiolabelled sulfate and utilizing isolation and detection techniques, this method enables the study of endocytic uptake and trans-Golgi network arrival of cargo proteins.
03:44
Generation of GFP-Tagged Recombinant Respiratory Syncytial Virus Using a T7 Expression System
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2026
Source: Bouillier, C. et. al., Amplification, and Titration of Recombinant Respiratory Syncytial Viruses. J. Vis. Exp. (2019)This video demonstrates the generation of a green fluorescent protein (GFP)-expressing recombinant respiratory syncytial virus using a T7-based reverse genetics system. The co-transfection of plasmids encoding viral proteins and a GFP-tagged genome leads to protein expression and initiates virus rescue.
FAQs
Binding of transferrin provides the trigger for following receptor movement through the cell. Fluorescence microscopy can reveal where the receptor is located at the membrane, when it is internalized, and how it later recycles or traffics through the cell. This sequence connects ligand recognition with membrane dynamics and intracellular transport, making uptake behavior directly observable.
The GFP moiety acts as a visual reporter, so the receptor’s location does not need to be inferred indirectly. Because the fluorescent signal is genetically linked to the receptor, changes in signal distribution can be used to examine localization, internalization, recycling, and trafficking. This also supports testing how receptor modifications or cellular conditions alter uptake.
These processes describe different stages of receptor behavior rather than one single uptake event. Researchers can determine whether transferrin binding is followed by movement away from the cell surface, return of the receptor through recycling, or altered intracellular trafficking. Separating these stages helps bioengineers evaluate how delivery systems exploit receptor-mediated endocytosis and transport.
The construct allows researchers to examine how receptor modifications or changes in the cellular environment affect receptor behavior. Fluorescence observations can reveal associated changes in localization, internalization, recycling, trafficking, and overall cellular uptake. This links molecular or environmental design changes to measurable membrane dynamics, which is useful when optimizing engineered cells or receptor-targeted delivery systems.
Cells containing the construct are examined by fluorescence microscopy, and the observed green signal is followed across the cell surface and inside the cell. Researchers use these patterns to assess receptor localization, internalization, recycling, and trafficking, then relate them to transferrin binding or to uptake by an engineered delivery system.
When a drug-delivery system or nanoparticle is designed to exploit receptor-mediated endocytosis, the reporter provides a way to examine receptor-associated uptake and trafficking. Fluorescence observations can show how the receptor behaves during interaction with the engineered system and whether cellular transport changes. This information helps bioengineers evaluate designs in relation to membrane dynamics and intracellular transport.