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Using an α-Bungarotoxin Binding Site Tag to Study GABA A Receptor Membrane Localization and Trafficking
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Chapters
- 00:05Title
- 02:08Preparation of Hippocampal Neuronal Cultures
- 03:13Endocytosis Assay
- 07:23Image Acquisition
- 08:26Results: Visualizing Surface GABAAR Localization and Endocytosis with Fluorescent α-Bungarotoxin
- 11:03Conclusion
Here we demonstrate the use of fluorescent Alexa dye coupled to α-bungarotoxin to measure GABA A receptor surface localization and endocytosis in hippocampal neurons. Through the use of constructs bearing a short extracellular tag that binds α-bungarotoxin, analysis of plasma membrane protein endocytic trafficking can be achieved.
